A biolistic process for in vitro gene transfer into chicken embryos
نویسندگان
چکیده
منابع مشابه
A biolistic process for in vitro gene transfer into chicken embryos.
Chicken embryos kept in culture medium were bombarded using a high helium gas pressure biolistic device. To optimize the factors that affect transformation efficiency, the lacZ gene under control of the human cytomegalovirus immediate early enhancer/promoter was used as a reporter gene. There was an inverse relationship between survival rate and transformation efficiency. The best conditions ob...
متن کاملEx ovo electroporation for gene transfer into older chicken embryos.
In ovo electroporation is an excellent method to ectopically induce or inhibit gene expression in chicken embryos and to study the in vivo function of genes during embryonic development. However, the application of electroporation in ovo to date is limited to an early stage of incubation (< stage 20). In older embryos (> stage 22), the vitelline and allantoic vessels have developed extensively ...
متن کاملGene transfer into older chicken embryos by ex ovo electroporation.
The chicken embryo provides an excellent model system for studying gene function and regulation during embryonic development. In ovo electroporation is a powerful method to over-express exogenous genes or down-regulate endogenous genes in vivo in chicken embryos(1). Different structures such as DNA plasmids encoding genes(2-4), small interfering RNA (siRNA) plasmids(5), small synthetic RNA olig...
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Birth of germline chimeras by transfer of chicken embryonic germ (EG) cells into recipient embryos.
This study reports for the first time the production of chicken germline chimeras by transfer of embryonic germ (EG) cells into recipient embryos of different strain. EG cells were established by the subculture of gonadal tissue cells retrieved from stage 28 White Leghorn (WL) embryos with I/I gene. During primary culture (P(0)), gonadal primordial germ cells (gPGCs) in the stromal cells began ...
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ژورنال
عنوان ژورنال: Brazilian Journal of Medical and Biological Research
سال: 2001
ISSN: 0100-879X
DOI: 10.1590/s0100-879x2001000900003